Cell Slicing Current

What if slice a is aligned to the coordinate system?

When we align Slice A ∗ to the coordinate system of Slice B, we wish to recover the shape of Slice B, which is similar to Slice A. Thus, ideally, we want T (Slice A ∗ |Slice B) to be as similar as possible to Slice A, where the one-to-one correspondence between Slice A ∗ and Slice A is known.

How to crop a slice based on a Y axis?

Next, we simply crop slices based only on the x or y axis with a hard threshold to generate partially overlapping slices, where the overlap ratio is fully controlled by the threshold, as shown in Figure S4 A iii). Taking the y-axis as an example, given the Slice A, Slice B and overlap ratio, we can obtain the cropped slices as follows:

Can a brain slice be used in vitro?

Classically, this technique is performed in vitro either on brain slices, freshly dissociated neurons, or on cell culture models 3. When performed on neurons in brain slices, this technique presents several advantages.

How long does it take to incubate slices?

Incubate the slices at 33°C for 60 min; then, keep the beaker with slices at room temperature. The slices are viable for at least 4 h. We start electrophysiology right after 60 min of incubation at 33°C.

How do you slice a brain?

Take out the brain from the skull with the help of a medical spatula and immediately transfer it into the cold aerated cutting solution. Leave it there for 1 min while preparing the cold vibratome platform for supergluing the brain. Brain slicing (max 5-10 min).

What are cell-cell interactions?

Beyond ligand-receptor interactions, the umbrella term “cell-cell interactions” encompasses a wide variety of mechanisms that leverage diverse molecules, such as ions, metabolites, and even direct genetic transfer that is common between prokaryotic cells.